Anthelmintic activity of Annona crassiflora leaves against Haemonchus contortus: part 1: in vitro inhibition of the hatchability and larval development

Autores

  • Marco Antonio Martins Caldeira Universidade Federal Rural de Pernambuco
  • Franciellen Morais-Costa Universidade Federal de Minas Gerais, Montes Claros-MG, Brasil
  • Kaike Magno Macêdo Universidade Federal de Minas Gerais, Montes Claros-MG, Brasil
  • Valdo Soares Martins Júnior Universidade Federal de Minas Gerais, Montes Claros-MG, Brasil
  • Dheison Emanuel Augusto Pereira Universidade Federal de Minas Gerais, Montes Claros-MG, Brasil
  • Ana Cláudia Maia Soares Universidade Estadual do Sudoeste da Bahia, Itapetinga-BA, Brasil
  • Isabela Parolis Martins Universidade Federal de Minas Gerais, Montes Claros-MG, Brasil.
  • Idael Matheus Goes Lopes Universidade Federal de Minas Gerais, Montes Claros-MG, Brasil.
  • Fernão Castro Braga Universidade Federal de Minas Gerais Federal, Belo Horizonte-MG, Brasil
  • Neide Judith Faria Oliveira Universidade Federal de Minas Gerais, Montes Claros-MG, Brasil.
  • Eduardo Robson Duarte Universidade Federal de Minas Gerais, Montes Claros-MG, Brasil.

DOI:

https://doi.org/10.26605/medvet-v13n2-3067

Palavras-chave:

Cerrado, nematode, flavonoids, quantitative coproculture.

Resumo

We evaluated the activity of Annona crassiflora leaves against Haemonchus contortus resistant to albendazol.  Aqueous (AE), ethanolic (EE) and ethyl acetate (EAE) extracts were produced and the predominant presence of flavonoids was observed in HPLC-DAD chromatograms. Initially we evaluated the larval development inhibition (LDI) of dry A. crassiflora leaf powder or its AE directly in fecal quantitative cultures. The efficacies of the extracts, with or without tannins, on egg hatching inhibition (EHI) were investigated reveling that the EE was the most effective (LC90 = 8.96 mg/mL). However, after tannin removal, AE showed the highest activity (LC90 = 4.27 mg/mL). In the LDI test, the LC90 of AE was < 6.25 mg/g of fecal culture and to leaf powder of leaves was 69.14 mg/g.  High efficacies of AE and EE for EHI were detected and the tannins were not the main active metabolites. The anthelmintic potential of this plant could be attributed to association between flavonoids and other metabolites.

Downloads

Não há dados estatísticos.

Referências

Alawa, C.B.I., Adamu, A.M., Gefu, J.O., Ajanusi, O.J., Abdu, P.A., Chiezey, N.P., Alawa, J.N., Bowman D.D. In vitro screening of two Nigerian medicinal plants (Vernonia amygdalina and Annona senegalensis) for anthelmintic activity. Veterinary Parasitology, 113(1): 73-81, 2003.

Alvares, C.A.; Stape, J.L.; Sentelhas, P.C.; Gonçalves, J.L.M.; Sparovek, G.; Köppen’s climate classification map for Brazil. Meteorologische Zeitschrift, 22(6): 711– 728, 2013.

Amarante, A.F.T.; Sales, R.O. Controle de endoparasitoses dos ovinos: uma revisão. Revista Brasileira de Higiene e Sanidade Animal, 1(2): 14-36, 2007.

Araya, H. Studies on annonaceous tetrahydrofuranic acetogenins from Annona squamosa L. seeds. National Institute for Agro-Environmental Sciences, 23, 77–149. 2004.

Bastos, G.A.; Fonseca, L.D.; Ferreira, A.V.P.; Costa, M.A.M.S.; Silva, M.L.F.; Vasconcelos V.O.; Sousa, R.M.; Duarte, E.R. Helminthiasis characterization and anthelmintic efficacy for ewes and lambs raised in tropical semiarid region. Tropical Animal Health and Production, 49(5): 937– 943, 2017.

Borges. C.C.L. Atividade in vitro de antihelmínticos sobre larvas infectantes de nematódeos gastrintestinais de caprinos, utilizando a técnica de coprocultura quantitativa (Ueno, 1995). Parasitología Latinoamericana, 58(3-4), 142–147. 2003.

Coles, G.C.; Bauer, C.; Borgsteede, F.H.; Geerts, S.; Klei, T.R.; Taylor, M.A.; Waller, P.J. World association for the advancement of veterinary parasitology (WAAVP) methods for the detection of anthelmintic resistance in nematodes of veterinary importance. Veterinary Parasitology, 44(1-2): 35–44, 1992.

Duarte, E.R.; Silva, R.B.; Vasconcelos, V.O.; Nogueira, F.A.; Oliveira, N.J.F. Diagnóstico do controle e perfil de sensibilidade de nematoides de ovinos ao albendazol e ao levamisol no norte de Minas Gerais. Pesquisa Veterinária Brasileira. 32, 147-152, 2012.

Ferreira, L.E.; Castro, P.M.N.; Chagas, A.C.S.; França, S.C.; Beleboni, R.O. In vitro anthelmintic activity of aqueous leaf extract of Annona muricata L (Annonaceae) against Haemonchus contortus from sheep. Experimental Parasitolology, 134(3): 327– 332. 2013.

Githiori, J.B.; Hoglund, J.; Waller, P.J. Ethnoveterinary plants preparations as livestock dewormers: practices, popular beliefs, pitfalls and prospects for the future. Animal Health Research Reveiews, 6(1): 91–103, 2005.

Gordon, H.McL.; Whitlock, A.V. A new technique for counting nematode eggs in sheep feces. Journal Council for Scientific and Industrial Research, 12(1): 50–52, 1939.

Hiermann, A.; Kartnig, T.H.; Azzam, S.M. Ein Beitrag zur quantitativen Bestimmung der Procyanidine in Crataegus. Scientia Pharmaceutica, 54: 331–337, 1986.

Kamaraj, C.; Rahumann, A.A. Efficacy of anthelmintic properties of medicinal plant extract against Haemonchus contortus. Research in Veterinary Science, 91(3): 400– 404, 2011.

Keith, R.K. The differentiation of infective larvae of some common nematode parasites of cattle. Australian Journal of Zoology, 1: 223–235, 1953.

Kerboeuf, D.; Riou, M.; Guégnard, F. Flavonoids and related compounds in parasitic disease control. Mini Reviews in Medicinal Chemistry, 8(2); 116–128, 2008.

Klongsiriwet, C.; Quijada, J.; Williams, A.R.; Mueller–Harvey, I.; Williamson, E.M.; Hoste, H. Synergistic inhibition of Haemonchus contortus exsheathment by flavonoid monomers and condensed tannins. International Journal for Parasitology: Drugs and Drug Resistance, 5(3): 127–134, 2015.

Mengistu, G.; Hoste, H.; Karonen, M.; Salminen, J.P.; Hendriks, W.H.; Pellikaan, W.F. The in vitro anthelmintic properties of browse plant species against Haemonchus contortus is determined by the polyphenol content and composition. Veterinary Parasitology, 237: 110-116, 2017.

Miller, C.M.; Waghorn, T.S.; Leathwick, D.M.; Candy, P.M.; Oliver, A.M.B.; Watson, T.G. The production cost of anthelmintic resistance in lambs. Veterinary Parasitology 186(3-4): 376–381, 2012.

Morais–Costa, F.; Bastos, G.A.; Soares, A.C.M.; Costa, E.G.L.; Vasconcelos, V.O.; Oliveira, N.J.F.; Braga, F.C.; Lima, W.S.; Duarte, E.R. In vitro and in vivo action of Piptadenia viridiflora (Kunth) Benth against Haemonchus contortus in sheep. Veterinary Parasitology, 223(15): 43–49, 2016.

Morais–Costa, F.; Soares, A.C.M.; Bastos, G.A.; Nunes, Y.R.; Geraseev, L.C.; Braga, F.C.; Lima, W.S.; Duarte, E.R. Plants of the Cerrado naturally selected by grazing sheep may have potential for inhibiting development of Haemonchus contortus larva. Tropical Animal Health and Production, 47(7): 1321–1328, 2015.

Nery, P.S.; Nogueira, F.A.; Martins, E.R.; Duarte, E.R. Effect of Anacardium humile on the larval development of gastrintestinal nematodes of sheep. Veterinary Parasitology, 171(3-4): 361-364, 2010.

Nogueira, F.A.; Nery, P.S.; Morais-Costa, F.; Oliveira, N.J.F.; Martins, E.R.; Duarte, E.R. Efficacy of aqueous extracts of Genipa Americana L. (Rubiaceae) in inhibiting larval development and eclosion of gastrointestinal nematodes of sheep. Journal of Applied Animal Research, 42: 356-360, 2014.

Nogueira, F.A.; Fonseca, L.D.; Silva, R.B.; Ferreira, A.V.P.; Nery, P.S.; Geraseev, L.C.; Duarte, ER. In vitro and in vivo efficacy of aqueous extract of Caryocar brasiliense Camb to control gastrointestinal nematodes in sheep. Parasitology Research, 111(1): 325– 330, 2012.

Nyman, U.; Joshi, P.; Madsen, L.B.; Pinstrup, M.; Rajasekharan, S.; George, V.; Pushpangadan, P. Ethnomedical information and in vitro screening forangiotensin-converting enzyme inhibition of plants utilized as traditionalmedicines in Gujarat, Rajasthan and Kerala (India). Journal Ethnopharmacology, 60(3): 247–263, 1998.

Oliveira, A.F.; Costa Junior, L.M.; Lima, A.S.; Silva, C.R.; Ribeiro, M.N.; Mesquista, J.W.; Rocha, C.Q.; Tangerina, M.M.; Vilegas, W. Anthelmintic activity of plant extracts from Brazilian savana. Veterinary Parasitology, 236: 121–127, 2017.

]Oliveira, N.J.F.; Duarte, E. R.; Geraseev, L.C.; Souza, P.N.S.; Souza, M.F.; Mairink, J.A.M.; Gonzaga Jayme, D.; Lago, L.A. Toxicidade de Annona crassiflora sobre ovinos em teste de campo: relato de caso. Veterinária e Zootecnia, 18: 622-624, 2011.

Raza, A.; Kopp, S.R.; Bagnall, N.H.; Jabbar, A.; Kotze, A.C. Effects of in vitro exposure to ivermectin and levamisole on the expression patterns of ABC transporters in Haemonchus contortus larvae. International Journal for Parasitology: Drugs and Drug Resistance, 6(2): 103–15, 2016.

Santos, D.Y.A.C.; Salatino, M.L.F. Foliar flavonoids of Annonaceae from Brazil: taxonomic significance. Phytochesmistry, 55(6): 567–573, 2000. Santos, J.M.L.; Monteiro, J.P.; Ribeiro, W.L.C.; Macedo, I.T.F.; Araujo-Filho, J.V.; Andre, W.P.P. High levels of benzimidazole resistance and β-tubulin isotype 1 SNP F167Y in Haemonchus contortus populations from Ceará State, Brazil. Journal Small Ruminants, 146: 48–52, 2017.

Downloads

Publicado

08-04-2020

Como Citar

Caldeira, M. A. M., Morais-Costa, F., Macêdo, K. M., Martins Júnior, V. S., Pereira, D. E. A., Soares, A. C. M., Martins, I. P., Lopes, I. M. G., Braga, F. C., Oliveira, N. J. F., & Duarte, E. R. (2020). Anthelmintic activity of Annona crassiflora leaves against Haemonchus contortus: part 1: in vitro inhibition of the hatchability and larval development. Medicina Veterinária, 13(2), 184–191. https://doi.org/10.26605/medvet-v13n2-3067

Edição

v. 13 n. 2 (2019)

Seção

Medicina Veterinária Preventiva

Licença

A Revista de Medicina Veterinária permite que o autor retenha os direitos de publicação sem restrições, utilizando para tal a licença  Creative Commons CC BY-NC-SA 4.0.

De acordo com os termos seguintes:

Atribuição — Você deve dar o crédito apropriado, prover um link para a licença e indicar se mudanças foram feitas. Você deve fazê-lo em qualquer circunstância razoável, mas de nenhuma maneira que sugira que o licenciante apoia você ou o seu uso.

NãoComercial — Você não pode usar o material para fins comerciais.

CompartilhaIgual — Se você remixar, transformar, ou criar a partir do material, tem de distribuir as suas contribuições sob a mesma licença que o original.

Sem restrições adicionais — Você não pode aplicar termos jurídicos ou medidas de caráter tecnológico que restrinjam legalmente outros de fazerem algo que a licença permita.