SUBSTRATES FOR THE PRODUCTION OF ENTOMOPATHOGENIC ISOLATES OF Fusarium caatingaense TO CONTROL Dactylopius opuntiae

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Palabras clave:

Biological control, conidia production and viability, mass production, vegetable substrates

Resumen

Opuntia ficus-indica (prickly pear) is used as a source of animal feed in Northeast Brazil. Its yield has been severely affected by insect pest Dactylopius opuntiae (cochineal scale). Fusarium caatingaense isolates have been reported to be effective in controlling this pest. Therefore, this study aimed to select substrates for the production of F. caatingaense. Conidia production and viability of five F. caatingaense isolates were evaluated on six substrates: rice, sugarcane bagasse, sugarcane bagasse + 0.66% w/v peptone, corn grains, corn grains + 0.66% w/v peptone, and sweet corn grains. Rice and corn were the best substrates for the production of most isolates. Isolate URM 6778 was selected for the analyses of sporulation, germination, and pathogenicity after storage in these substrates at 4 ºC and 28 ºC and periods of 0, 15, 30, and 60 days. Corn was the best substrate for the fungus and 4 ºC was the most suitable storage temperature. The highest conidia concentration was observed on the 30th day of storage, with a decrease on the 60th day. Conidia remained viable in all periods analyzed, except on the 60th day at 28 ºC. Pathogenicity against D. opuntiae was kept until 30 days of storage.

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Publicado

2023-02-07 — Actualizado el 2023-02-07

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Leão Soares de Oliveira, R., da Silva Santos, A. C. ., Félix da Costa, A., & Vieira Tiago, P. (2023). SUBSTRATES FOR THE PRODUCTION OF ENTOMOPATHOGENIC ISOLATES OF Fusarium caatingaense TO CONTROL Dactylopius opuntiae. Anais Da Academia Pernambucana De Ciência Agronômica, 19(2). Recuperado a partir de https://www.journals.ufrpe.br/index.php/apca/article/view/4936