Automated impedance-based and manual leukocyte differential counts in healthy equines

Autores

  • Maria Fernanda Cazini Silva Department of Veterinary Medicine, Roque Quagliato Veterinary Hospital, University Center of the Integrated Faculties of Ourinhos (Unifio), Ourinhos-SP, Brazil https://orcid.org/0009-0003-5300-0987
  • Luma Maria Rovina Brito Department of Veterinary Medicine, Roque Quagliato Veterinary Hospital, University Center of the Integrated Faculties of Ourinhos (Unifio), Ourinhos-SP, Brazil https://orcid.org/0009-0005-4836-6540
  • Beatriz de Azevedo Martins Department of Veterinary Medicine, Roque Quagliato Veterinary Hospital, University Center of the Integrated Faculties of Ourinhos (Unifio), Ourinhos-SP, Brazil https://orcid.org/0000-0002-5746-4303
  • Laryssa Eduarda de Campos Lopes Department of Veterinary Medicine, Roque Quagliato Veterinary Hospital, University Center of the Integrated Faculties of Ourinhos (Unifio), Ourinhos-SP, Brazil https://orcid.org/0000-0002-0119-1043
  • Yasmin Cunha Duarte Department of Veterinary Medicine, Roque Quagliato Veterinary Hospital, University Center of the Integrated Faculties of Ourinhos (Unifio), Ourinhos-SP, Brazil https://orcid.org/0000-0002-0015-6481
  • Matheus Nogueira Morais Department of Veterinary Medicine, Roque Quagliato Veterinary Hospital, University Center of the Integrated Faculties of Ourinhos (Unifio), Ourinhos-SP, Brazil https://orcid.org/0000-0002-7106-2538
  • Laura Arduino Vasconcelos Department of Veterinary Medicine, Roque Quagliato Veterinary Hospital, University Center of the Integrated Faculties of Ourinhos (Unifio), Ourinhos-SP, Brazil https://orcid.org/0000-0001-5542-6656
  • Mariana Orlandini Mendonça Ourinhos Veterinary Hospital (HVO), Ourinhos-SP, Brazil https://orcid.org/0000-0001-8394-0521
  • Tainara de Oliveira Martins Canguru Veterinary Hospital, Ourinhos-SP, Brazil https://orcid.org/0009-0005-4684-3446
  • Daniela Fernandez Montechiesi Autonomous Veterinary, Ourinhos-SP, Brazil https://orcid.org/0009-0000-0419-3303
  • Breno Fernando Martins de Almeida Department of Veterinary Medicine, Roque Quagliato Veterinary Hospital, University Center of the Integrated Faculties of Ourinhos (Unifio), Ourinhos-SP, Brazil. Faculty of Veterinary Medicine of Araçatuba, São Paulo State University, (FMVA-Unesp), Araçatuba-SP, Brazil https://orcid.org/0000-0002-6379-6734

DOI:

https://doi.org/10.26605/medvet-v17n4-5897

Palavras-chave:

horse, leukogram, impedance

Resumo

The speed and convenience provided by automated cell counters are reasons for the increasing use of this technology in veterinary practice, with impedance methodology being one of the most commonly used in Brazil. In this regard, the objective of this study was to compare the automated differential leukocyte count obtained by impedance with the manual count obtained from the evaluation of blood smears in healthy horses. For this purpose, the automated differential leukocyte count by impedance using a veterinary cell counter (Abx Micros ESV 60, Horiba) was compared to the manual count using optical microscopy in 545 blood samples from healthy horses. Paired t-tests or Wilcoxon tests, Deming regression, Pearson or Spearman correlations, and Bland-Altman analysis were used, considering differences significant when p<0.05. All optical microscopy counts were carried out by the same veterinary clinical pathologist with ten years of previous experience in the area. The automated method showed higher concentrations of segmented neutrophils and monocytes, and lower concentrations of lymphocytes and eosinophils compared to the manual method. The mean error of the automated count was -10.26% for segmented neutrophils, 11.04% for lymphocytes, -41.39% for monocytes, and -10.84% for eosinophils, ranging from -200% to 161.4% depending on the cell type. There was a significant correlation between the methodologies only for the counts of segmented neutrophils, lymphocytes, and eosinophils. In conclusion, the manual differential leukocyte count in healthy horses cannot be replaced by the automated method, and the analysis of blood smears remains a fundamental tool for the proper interpretation of the leukogram in horses.

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Referências

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Publicado

28-12-2023

Como Citar

Silva , M. F. C., Brito, L. M. R., Martins, B. de A., Lopes, L. E. de C., Duarte, Y. C., Morais, M. N., Vasconcelos, L. A., Mendonça, M. O., Martins, T. de O., Montechiesi, D. F., & Martins de Almeida, B. F. (2023). Automated impedance-based and manual leukocyte differential counts in healthy equines. Medicina Veterinária, 17(4), 249–256. https://doi.org/10.26605/medvet-v17n4-5897

Edição

v. 17 n. 4 (2023): Medicina Veterinária

Seção

Patologia Animal

Licença

Copyright (c) 2023 Maria Fernanda Cazini Silva , Luma Maria Rovina Brito, Beatriz de Azevedo Martins, Laryssa Eduarda de Campos Lopes, Yasmin Cunha Duarte, Matheus Nogueira Morais, Laura Arduino Vasconcelos, Mariana Orlandini Mendonça, Tainara de Oliveira Martins, Daniela Fernandez Montechiesi, Breno Fernando Martins de Almeida

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