Physicochemical and microbiological evaluation of sausages produced by conventional smoking and liquid smoke

Authors

DOI:

https://doi.org/10.26605/medvet-v19n4-7339

Keywords:

pork, preservation methods, meat products

Abstract

Smoking is an ancient practice for preserving food, providing aroma, color, flavor, and texture. Traditionally, it involves exposure to wood smoke, but this may contain toxic compounds and hinder product standardization. As an alternative, the meat industry has adopted liquid smoke, whose effects are still poorly studied. This study evaluated the physicochemical and microbiological characteristics of sausages smoked conventionally (CS), with liquid smoke (LS), and without smoking (NS). Three batches of sausages, formulated with pork, lard, and seasonings, were processed at 80°C for 3 hours. Microbiological analyses included coliforms, mesophilic and psychrotrophic aerobes, lactic acid bacteria (LAB), coagulase-positive Staphylococcus counts, and the detection of Salmonella. Physicochemical analyses measured protein, fat, moisture, ash, and pH. There were no significant differences between treatments regarding physicochemical and microbiological characteristics, indicating that smoking, whether conventional or with liquid smoke, does not alter sausage composition. In non-smoked sausages, coliforms at 30°C and LAB counts were found above the minimum detection limit of the technique. Liquid smoking proved to be a viable alternative to conventional smoking, maintaining the quality and safety of sausages.

Downloads

Download data is not yet available.

References

Aaslyng, M.D.; Koch, A.G. The use of smoke as a strategy for masking boar taint in sausages and bacon. Food Research International, 108: 387-395, 2018.

Abd El Aziz, N.A.; El-Razek, A.; Mohamed, A. Effect of different smoking methods on quality and safety of beef meat. Egyptian Journal of Food Science, 49(1): 1-17, 2021.

Afshin, J.; Saied, S.; Babak, A. Microbial properties of hot smoked sausage during shelf life. Global Veterinaria, 7(5): 423-426, 2011.

Andrews, W.H. et al. Salmonella. In: Downes, F.P.; Ito, K. Compendium of Methods for the Microbiological Examination of Foods. 4th ed. Washington, DC: American Public Health Association, 2001. p.357-376.

AOAC. Association of Official Agricultural Chemists. Official Methods of Analysis of the AOAC. 16th ed. Arlington: AOAC International, 2011. v.1, p.4-30.

Bohrer, B.M. Nutrient density and nutritional value of meat products and non-meat foods high in protein. Trends in Food Science & Technology, 65: 103-112, 2017.

Bhuyan, D.; Shahariar, S.M.; Mohsin, M. Effect of different smoking methods on the quality of pork sausages. Veterinary World, 11(12): 1712-1718, 2018.

BRASIL. Ministério da Agricultura, Pecuária e Abastecimento. Instrução Normativa Nº 4, de 31 de março de 2000. Disponível em: https://pesquisa.in.gov.br/imprensa/jsp/visualiza/index.jsp?data=05/04/2000&jornal=1&pagina=54&totalArquivos=73. Acesso em: 24 set. 2024.

BRASIL. Ministério da Saúde. Instrução Normativa Nº 313, de 4 de setembro de 2024. Disponível em: https://www.in.gov.br/en/web/dou/-/instrucao-normativa-n-313-de-4-de-setembro-de-2024-582631446. Acesso em: 11 jun. 2025.

Carballo, J. Sausages: Nutrition, safety, processing and quality improvement. Foods, 10(4): 890, 2021.

Charão, G.N. et al. Level of compliance with good manufacturing practices and microbiological profile of mixed fresh sausages. Ciência Animal Brasileira, 25: e-76582E, 2024.

Daniel, G.T.; Martins, M.T.; De Almeida, J.P. Determination of pathogens and contamination indicators in fresh pork sausages in the state of São Paulo. Semina: Ciências Biológicas e da Saúde, 44(1): 89-96, 2023.

Ernawati, E. et al. Physical and chemical characterization of African catfish smoked sausage with different liquid smoke concentrations and immersion durations. In: E3S Web of Conferences. Vol. 322, p. 04001, 2021. EDP Sciences.

Franz, C.M.A.P.; Von Holy, A. Thermotolerance of meat spoilage lactic acid bacteria and their inactivation in vacuum-packaged Vienna sausages. International Journal of Food Microbiology, 29(1): 59-73, 1996.

Gómez, I. et al. The effects of processing and preservation technologies on meat quality: Sensory and nutritional aspects. Foods, 9(10): 1416, 2020.

Helrich, K. Standard solutions and certified reference. In: Official methods of analysis of the Association of Official Analytical Chemists: food composition: additives: natural contaminants. 15th ed. Arlington: Association of Official Analytical Chemists, 1990. v.2, p.640-641.

IDF. International Dairy Federation. Yogurt: Enumeration of characteristic microorganisms colony count technique at 37°C. IDF Standard 117A. Brussels: International Dairy Federation, 1988.

Kobylyatsky, P.S. et al. Production of raw smoked beef products using lactobacilli. International research conference on Challenges and Advances in Farming, Food Manufacturing, Agricultural Research and Education, KnE Life Sciences. 2021: 272-280, 2021.

Kornacki, J.L.; Johnson, J.L. Enterobacteriaceae, coliforms and Escherichia coli as quality and safety indicators. In: Downes, F.P.; Ito, K. Compendium of Methods for the Microbiological Examination of Foods. 4th ed. Washington, DC: American Public Health Association, 2001. p.69-80.

Lancette, G.A.; Tatini, S.R. Staphylococcus aureus. In: Downes, F.P.; Ito, K. Compendium of Methods for the Microbiological Examination of Foods. 4th ed. Washington, DC: American Public Health Association, 2001. p.387-400.

Mann, E. et al. Psychrophile spoilers dominate the bacterial microbiome in musculature samples of slaughter pigs. Meat Science, 117: 36-40, 2016.

Merck. Reactivos, Diagnóstica, Productos Químicos 1992/93. Darmstadt: Merck, 1993.

Microsoft Corporation. Microsoft Excel (Versão 2019). Redmond: Microsoft Corporation, 2018. Disponível em: https://www.microsoft.com/. Acesso em: 10 jun. 2025.

Morton, R.D. Aerobic plate count. In: Downes, F.P.; Ito, K. Compendium of Methods for the Microbiological Examination of Foods. 4th ed. Washington, DC: American Public Health Association, 2001. p. 63-67.

Özpolat, E.; Patir, B. Determination of shelf life for sausages produced from some freshwater fish using two different smoking methods. Journal of Food Safety, 36(1): 69-76, 2016.

Pothakos, V. et al. Lactic acid bacteria and their controversial role in fresh meat spoilage. Meat Science, 109: 66-74, 2015.

Racioppo, A. et al. Use of Food Spoilage and Safety Predictor for an “A Priori” Modeling of the Growth of Lactic Acid Bacteria in Fermented Smoked Fish Products. Foods, 11(7): 946, 2022.

Saha, A.; Birkeland, S.; Løvdal, T. The effect of K-lactate salt and liquid smoke on bacterial growth in a model system. Journal of Aquatic Food Product Technology, 26(2): 192-204, 2017.

Shaltout, F.A.; Salem, A.M.; Khater, D.F.; Lela, R.A. Studies on bacteriological profile of some meat products. Benha Veterinary Medical Journal, 31(1): 43-49, 2016.

Shao, L. et al. Advances in understanding the predominance, phenotypes, and mechanisms of bacteria related to meat spoilage. Trends in Food Science & Technology, 118: 822-832, 2021.

Škaljac, S. et al. Influence of collagen and natural casings on the polycyclic aromatic hydrocarbons in traditional dry fermented sausage (Petrovská klobása) from Serbia. International Journal of Food Properties, 21(1): 667-673, 2018.

Takeda, S. et al. Functionality of liquid smoke as an antimicrobial in cooked meat products: Liquid smoke suppresses spoilage-related lactic acid bacteria. Food Science and Technology Research, 27(5): 759-768, 2021.

University of Amsterdam. JASP (Versão 0.18.0.0). Amsterdam: JASP Team, 2023. Disponível em: https://jasp-stats.org/. Acesso em: 10 jun. 2025.

Winarni, I.; Komarayati, S. A review: The utilization and its benefits of liquid smoke from lignocellulosic waste. In: IOP Conference Series: Earth and Environmental Science, 914(1): 012068, 2021.

Yalçin, M.Y.; ?eker, M. Effect of salt and moisture content reduction on physical and microbiological properties of salted, pressed and freeze dried turkey meat. LWT-Food Science and Technology, 68: 153-159, 2016.

Zeng, X. et al. Effects of liquid smoking on the microbiological and physicochemical characteristics of Suan Yu, a traditional Chinese fermented fish product. Journal of Aquatic Food Product Technology, 30(3): 296-314, 2021.

Zhang, L. et al. Effect of different types of smoking materials on the flavor, heterocyclic aromatic amines, and sensory property of smoked chicken drumsticks. Food Chemistry, 367: 130680, 2022.

Published

2025-12-31

How to Cite

Werneck, J. G., Souza, A. H. de, Costa, D. A., Garrido, N. F., & Valente, G. L. C. (2025). Physicochemical and microbiological evaluation of sausages produced by conventional smoking and liquid smoke. Medicina Veterinária, 19(4), 373–380. https://doi.org/10.26605/medvet-v19n4-7339

Issue

Section

Technology and Inspection of Products of Animal Origin

Similar Articles

1 2 > >> 

You may also start an advanced similarity search for this article.